General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Parathyroid hormone (PTH) is recognized by three different class B GPCRs; PTH1, PTH2, and PTH3, which couple to Gs to stimulate cAMP production. The PTH1 receptor is found in high levels in the kidney and bone, where it binds PTH and PTHrP to regulate Ca2+ homeostasis (Brown et al, 1996). The PTH2 receptor, which binds PTH and the neuropeptide tuberoinfundibular peptide 39 (TIP-39) but not PTHrP, has about 50% amino acid sequence identity to the PTH1 receptor. PTH2 is found in the greatest volume in the nervous system and at a very low density in the kidney and bone (Usdin et al, 1995). The PTH3 receptor, which has been recently discovered in the zebrafish, has 61% amino acid identity to the zebrafish PTH1 receptor and also seems to share its ligand affinity (Rubin et al, 1999). Studies with the selective PTH2 receptor agonist, TIP-39, and the distribution of the receptor in the superficial dorsal horn of the spinal cord suggest the receptor may play a role in pain perception (Usdin et al, 1999). Millipore′s cloned human PTH2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PTH2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at PTH2.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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