General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Secretin, a member of the secretin-glucagon peptide hormone family, is a 27 amino acid peptide that was originally isolated from the duodenum. In the duodenum, secretin is released to stimulate the release of digestive juices in the pancreas (Bayliss et al, 1902). The receptor for secretin is a class 2 (or class B) G protein coupled receptor that signals through Gs to stimulate cAMP production (Dong et al, 2002). Along with its traditional role in the pancreas, studies in secretin-deficient mice have shown miscommunication between the CA3 Schaffer collateral and CA1 pyramidal neurons, causing a deficiency in synaptic transmission (Nishijima et al, 2006). This miscommunication of CA1 dendrites is found in Autism, Rett Syndrome, and most forms of mental retardation, suggesting secretin could be a potential target for treatment of these disorders. Millipore′s cloned human Secretin-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Secretin expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at Secretin.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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