General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following overnight recovery, assays for calcium response.
The orexin/hypocretin peptides orexin A and orexin B (also known as hcrt-1 and hcrt-2) are 33- and 28-amino acid peptides, respectively, that are preferentially expressed in hypothalamus. The orexins bind to two GPCRs, OX1 and OX2, to increase intracellular calcium. Experiments with OX1 selective antagonists have shown the receptor plays a role in food consumption and also in acquisition and consolidation of spatial memory (Haynes et al., 2000; Akbari et al, 2006). In addition, the orexinergic system plays a role in energy metabolism and stress responses (Kukkonen et al., 2002). Millipore′s cloned human OX1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OX1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between the OX1 and its ligands.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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