General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
GPR103 is a Gq-coupled receptor that is located in the brain and adrenal gland, and is activated by a 43-amino acid RFamide peptide, QRFP43, and a 26 amino acid fragment, QRFP26/26RFa/Peptide 518 (Jiang et al., 2003; Fukusumi et al., 2003). QRFP43 is the active form in rat brain, and intracerebral administration of QRFP43 induces feeding and metabolic rate (Takayasu et al., 2006; Moriya et al., 2006). Millipore′s cloned human GPR103-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR103 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at GPR103.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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