General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptor family of G protein-coupled receptors. Five NPY receptors, Y1, Y2, Y4, Y5 and y6, have been defined at the molecular level, and each signals primarily through Gi/o. Binding of NPY family peptides to NPY receptors mediates a variety of physiological effects, including promotion of food intake, decreased anxiety, inhibition of neurotransmitter and hormone release, vasoconstriction, and gut motility. Y4 binds preferentially to PP, with significant binding to NPY and PYY (Michel et al., 1998). Y4 plays a role in control of reproduction, as Y4-null, ob/ob double knockout mice have increased fertility relative to ob/ob mice with functional Y4 (Sainsbury et al., 2002). In addition, mice lacking Y4 display resistance to weight gain induced by high fat diet, and mice lacking both Y2 and Y4 are resistant to the enhanced weight gain exhibited by Y1-null mice fed a high fat diet (Sainsbury et al., 2006). Millipore′s cloned human Y4-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Y4 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at Y4 Receptor.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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