General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
The P2Y GPCRs serve as receptors for extracellular nucleotides (Abbracchio et al., 2006). P2Y2 (also known as P2U) is activated by UTP and ATP, and couples to Gq to increase intracellular calcium (Parr et al., 1994). The P2Y2 receptor knockout mouse displays defective nucleotide-stimulated Cl- secretion, and a P2Y2 selective agonist increases tracheal epithelial chloride and water secretion (Cressman et al., 1999; Yerxa et al., 2002). As a result, P2Y2 is a potential target for treatment of cystic fibrosis. In addition, the P2Y2 KO mouse displays salt-resistant hypertension (Rieg et al., 2007). Millipore′s cloned human P2Y2-expressing cell line is made in the 1321N1host, which supports high levels of recombinant P2Y2 expression on the cell surface. Thus, the cell line is an ideal tool for screening for agonists and antagonists of P2Y2.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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