Merck, I3146-5ML, ITS Liquid Media Supplement (100×)

Application
ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
α mouse liver 12 (AML-12) cells
rat fetal testis explants
human dental pulp stem cells (DPSC)

Biochem/physiol Actions
Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
Transferrin: an iron-transport protein.
Selenium: an essential trace element normally provided by serum.

Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Other Notes
Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.