Merck, IM1014-50UG, Anti-Matriptase/MT-SP1 Rabbit pAb

General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~75 kDa matriptase protein.
Recognizes the ~75 kDa matriptase protein in MCF-7 cells.
This Anti-Matriptase/MT-SP1 Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of Matriptase/MT-SP1.
Immunogen
Human
a synthetic peptide corresponding to amino acids near the C-terminus of human matriptase
Application

Immunoblotting (1:500-1:2000)
Immunoprecipitation (5-20 g/mg lysate)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In Tris-citrate/phosphate buffer, pH 7.0-8.0.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Analysis Note
Positive Control
MCF-7 cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
Santin, A.D., et al. 2003. Cancer98, 1898.
Takeuchi, T., et al. 2000. J. Biol. Chem.275, 26333.
Lin, C.Y., et al. 1999. J. Biol. Chem.274, 18231.
Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 11054.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic (H)