Merck, IM57-100UG, Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7)

General description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa MTI-MMP protein in overexpressing cells.
Recognizes the ~60 kDa MTI-MMP protein in HT-1080 cells and in ConA-treated MD-MB-231 cells.
This Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MT1-MMP (Ab-4).
Immunogen
Human
a synthetic peptide (CDGNFDTVAMLRGEM) corresponding to amino acids 319-333 in the hemopexin-like domain of human MT1-MMP
Application

Immunoblotting (10 g/ml)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HT-1080 or ConA-treated MD-MB-231 cells
Other Notes
Sakakibara, M., et al. 1999. Cancer85, 231-239.
Mattei, M.G., et al. 1997. Genomics:40, 168.
Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
Ueno, H., et al. 1997. Cancer Res.57, 2055.
Strongin, A.Y., et al. 1995. J. Biol. Chem.270 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Cottam, D. W. and Rees, R. C., 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J. F., 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Goldberg, G.I., et al. 1989. PNAS86, 8207.
This antibody cross-reacts slightly with human MMP-3. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. Antibody should be titrated for optimal results in individual systems.
Legal Information
Manufactured by Daiichi Fine Chemicals C0., Ltd., Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)