Description
General description
Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.
Application
Krebs-Henseleit Buffer Modified has been used:
to collect the rat brains for ex vivo brain slice experiments
to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
to collect the uterine and forelimb blood vessel samples from horses
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.1 g/L sodium bicarbonate.