Merck, KMM-101NVS-0.25ML, KOD One™ PCR Master Mix

General description
KOD One™ PCR master Mix is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/kb for template DNA Genomic DNA amplification
cDNA amplification
Direct PCR
Colony PCR
Amplification of NGS libraries
Site direct gene mutation

Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA
Features and Benefits
Fast: Amplifies the targets using the following very short conditions:
o =1 kb: 1 sec
o 1~ 10 kb: 5 sec/ kb
o 10 kb~: 10 sec/ kb
Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2.
High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)

Packaging
KMM-101NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
Other Notes
For R&D use only. Not for drug, household, or other uses.
Legal Information
*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.