Description
General description
KOD One™ PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/ kb for template DNA Application
Genomic DNA amplification
cDNA amplification
Direct PCR
Colony PCR
Amplification of NGS libraries
Site direct gene mutation
KOD One™ PCR Master Mix - Blue has been successfully utilized directly on crude samples, including unextracted and purified milk, using alkaline lysis in asymmetric PCR, demonstrating the efficacy and robustness of KOD DNA Polymerase-based molecular detection methods
Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA
Features and Benefits
Fast: Amplifies the targets using the following very short conditions:
o =1 kb: 1 sec
o 1∼ 10 kb: 5 sec/ kb
o 10 kb∼: 10 sec/ kb
Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2. KOD One PCR Master Mix Blue includes a loading dye (BPB) to allow direct loading onto agarose gels.
High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase. This mix can be used for various purposes where this would be an advantage (e.g., in the preparation of long target amplicons for sequencing).
High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)
Packaging
KMM-201NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-201NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
Preparation Note
Stored at -20 °C for a year. 4 °C for a month.
Other Notes
For R&D use only. Not for drug, household, or other uses.
Legal Information
*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.
