Description
General description
BAC-Optimised Replicator™ v2.0 Electrocompetent Cells are designed for BAC library construction or trasformation of large constructs. These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of Biosearch Technologies Cloning Kits. Packed as DUOs (2 transformations per tube)
Application
Cloning
BAC Library construction
Cloning of large constructs
Features and Benefits
Highest available transformation efficiencies for library construction and screening
Ideal for BAC library construction or transformation of large inserts
Optimised for high yields of DNA
Transformation efficiency: ≥ 1 x 1010 (cfu/µg pUC or pKanR DNA))
Genotype BAC-Optimized Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)
Components:
BAC-Optimized Replicator v2.0 Electrocompetent Cells
Transformation Control pKanR
Arabinose Induction Solution
Recovery Media
Legal Information
CopyRight is a registered trademark of Lucigen Corporation
Replicator is a trademark of Lucigen Corporation
pEZ is a trademark of Lucigen Corporation
pSMART is a registered trademark of Lucigen Corporation