Merck, LGC603001-1KIT, E. cloni EXPRESS BL21(DE3) Electrocompetent Cells (DUOs)

General description
E. cloni EXPRESS BL21(DE3) Electrocompetent Cells offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment.
Application
Cloning, routine protein expression
Features and Benefits
Routine protein expression
High transformation efficiency
Short protein expression protocol time

Transformation efficiency: ≥ 5 x 109 (cfu/µg)

Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Components:

E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells

pUC 19 Trasformation Control

Recovery Media