Merck, LGC604012-1KIT, E. cloni EXPRESS BL21(DE3) Chemically Competent Cells (DUOs)

Name: E. cloni EXPRESS BL21(DE3) Chemically Competent Cells (DUOs)
Brand: Merck
SKU: LGC604012-1KIT

Size:12 x 80 μL, Competent Cells for protein expression, DNA cloning, DNA transformation, Heat shock transformation, competent E.Coli, pkg of 24 reactions (12 x 80 µL), Cfu/µg ≥ 1 x 10⁷

Catalog Number: LGC604012-1KIT

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Category

Cell-Free Protein Expression Kits

Pack Size1 Kit

TagsLabware Labware Partners LGC Biosearch™ Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
E. cloni EXPRESS BL21(DE3) Chemically Competent Cells are ideal for routine protein expression applications. These strain carries a chromosomal copy of the T7 RNA Polymerase gene under the control of the lacUV5 promoter and it is suitable for production of protein from target genes cloned into T7 driven expression vectors.
Application
Cloning, routine protein expression
Features and Benefits
Routine protein expression
High transformation efficiency
Short protein expression protocol time

Transformation efficiency: ≥ 1 x 107 (cfu/µg pUC19)

Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Components:

E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells

pUC 19 Trasformation Control

Recovery Media

Specifications

General

UNSPSC Code	41106202
NACRES	NB.43
biological source	Escherichia coli
manufacturer/tradename	Lucigen 60401-2
packaging	pkg of 24 reactions (12 x 80 µL)
growth mode	growth or suspension
technique(s)	microbiological culture: suitable
cell transformation	competent cell type: chemically competenttransformation efficiency: 1×107 cfu/μg
shipped in	dry ice
storage temp.	-65 to -96°C

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