Merck, LGC605141-1KIT, MC1061 F- Electrocompetent Cells, (DUOs)

General description
MC1061 F- is a non amber-suppressing strain that is commonly used for general cloning and Phage display because of its ability to reach very high transformation efficiencies. It is identical to Lucigen strain SS320, except that it lacks the F’ episome that is required for infection by filamentous phage such as M13. The strain can be used for plasmid propagation and phage display, but cannot be used for reinfection by filamentous phage.
Application
Phage Display Libraries, routine cloning, protein expression
Features and Benefits
Perfect for antibody phage display library creation
Lacks F’ episome, not suitable for M13 phage display
Create larger libraries; speed recovery
Supports cloning of methylated DNA, blue-white screening capable
Transformation efficiency: ≥ 4 x 1010 (cfu/µg pUC19)

Genotype: MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1

Components:

MC1061 F- Electrocompetent Cells

pUC 19 Trasformation Control

Recovery Media