Merck, LGCQEF81050-50ML, QuickExtract FFPE DNA Extraction Kit

General description
The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process. These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.

The QuickExtract FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR
Application
Isolation of DNA from FFPE samples for PCR-based analysis, e.g., microsatellite detection, SNP detection, tumour heterogeneity studies, copy number detection, methylation analysis, and Short Tandem Repeat (STR) analysis
Features and Benefits

• Simple, rapid extraction of PCR-ready DNA from challenging FFPE samples
• Fast: PCR-ready DNA in minutes, not days
• Simple: No spin columns or transfer steps which helps increase yields
• Compatible: Extracted DNA is compatible with both real-time and end-point PCR
• Safe: No xylene or phenol extractions used