Merck, LGCQER090150-50ML, QuickExtract RNA Extraction Kit

General description
The QuickExtract RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis. The result is easy processing of one to hundreds of samples in minutes, with no sample loss or toxic organic solvents. The QuickExtract RNA Extraction Solution works with cultured adherent and suspension cells including buccal cells, and has been tested on human, mouse, rat, E. coli, and S. aureus cell cultures. It is not suitable for tissue samples or plant samples. An optional DNase I treatment may improve certain downstream applications.
Application
Preparation of RNA from cultured adherent and suspension cells for RT-PCR.
Features and Benefits

• Rapid extraction of RT-PCR-ready RNA from cultured mammalian cells
• Fast: Extract end-point and real-time RT-PCR ready RNA in minutes from low to high numbers of samples
• Simple: single tube protocol
• Compatible: Extracted RNA is compatible with both real-time and end-point RT-PCR
• Automation-friendly: Simple protocol makes incorporation into an automated workflow easy
• Flexible: A DNase I digestion step can be added for sensitive downstream applications when necessary
• Safe: No toxic reagents used