Merck, M2178-500G, Mycoplasma agar base

Application
Used for isolation and cultivation of mycoplasma.
Preparation Note
Suspend 36 g of Mycoplasma Agar Base in 700 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add a 300 ml 1% solution of Horse Serum to the agar base or add 10 vials of Mycoplasma Enrichment Supplement (Cat. No. M6930) to the Mycoplasma Agar Base. Mix well before dispensing. Dispense into petri plates or tubes.
Other Notes
Ingredients (g/L)
Beef heart, infusion, 250.00
Peptic digest of animal tissue, 10.00
Sodium chloride, 5.00
Agar, 15.00
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany