Description
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used to:
maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation
to rinse the aspiration line during the collection of the ovum from German Holstein cows
to culture cardiac endothelial cells from rats
Preparation Note
Formulated to contain 14.7 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.