Merck, M3817-500ML, MegaCell™ RPMI-1640 Medium

General description
MegaCell™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.
Application
MegaCell™ RPMI-1640 Medium has been used:
as a component of the TSC medium for culturing trophoblast stem cells (TSC)
as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs

Preparation Note
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
Legal Information
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.