Merck, MAB1048, Anti-Bone Morphogenetic Protein 6 Antibody, clone Morph-6.1

General description
45/18 kDa
FUNCTION: SwissProt: P22004 # Induces cartilage and bone formation.
SIZE: 513 amino acids; 57226 Da
SUBUNIT: Interacts with SOSTDC1 (By similarity).
SUBCELLULAR LOCATION: Secreted (By similarity).
SIMILARITY: SwissProt: P22004 ## Belongs to the TGF-beta family.
Synthetic peptide vgr 1-29C-KLH.
Application
Immunohistochemistry: 1-5 μg/mL. Fixation: formaldehyde, paraffin. Tissue pretreatment: microwave treatment (6 x 5) min is required.

Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Bone morphogenetic protein 6 (BMP-6) is a member of the TGF-b superfamily of cytokines regulating homeotic gene expression, embryonic development and neurogenesis. MAB1048 recognizes BMP-6. Pre-incubation of MAB1048 with amino-terminal peptide 1-29 of BMP-6 inhibits binding of the monoclonal to tissue sections. Pre-incubation with other amino-terminal synthetic peptides of human BMP-7, BMP-4, BMP-3 and BMP-2 as well as irrelevant peptides does not inhibit specific tissue staining. The antigen is highly conserved phylogenetically and is thus anticipated to react with most mammalian species.
Physical form
Purified immunoglobulin from culture supernatant. Lyophilized from PBS containing 5 mg/mL BSA and 0.05% Kathon as a preservative. Reconstitute with 0.5 mL of sterile distilled water.
Preparation Note
Maintain lyophilized material at 2-8°C for up to 12 months after date of receipt. After reconstitution maintain at -20*C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Embryonic and adult central nervous system, rat radial glial cells, fetal intestine, healing skin or chondrocytes
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.