Description
General description
90-100 kD
Monocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations.
Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
Immunogen
Rat spleen cells
Application
Immunohistology on frozen sections at 1:50-1:300. Paraffin embedded tissue (Bouin fixation): 1:50-1:300 (requires pretreatment with trypsin or pronase).
Flow cytometry at 1:100 - 1:200. Use 10 μL of this working dilution to label 10E6 cells.
Radioimmunoassay
Also suitable for Immunoprecipitation, and Western Blotting.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Macrophages/Monocytes Antibody, clone ED-1 is validated for use in Flow Cytometry (FC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), and Western Blotting (WB) for the detection of CD68.
Biochem/physiol Actions
Recognizes a single chain glycoprotein of 90-100 kD that is expressed on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen has many characteristics in common with macrosialin in the mouse and CD68 in the human.
Clone ED1, which recognises a CD68 like molecule in rat is an excellent marker of activated microglia. For pan microglial markers, staining both resting and activated microglia, use like CD11b (MAB1387Z and others) or iba-1 (MABN92).
Physical form
Format: Purified
Protein A purified.
Purified mouse IgG1 in PBS (pH 7.4) and 0.09% sodium azide
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Macrophages, monocytes, lymphoid organs
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL1511; CBL260
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
