Description
General description
BIOCHEMISTRY:
p I = 7.0
Immunogen
Raji cells
Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Human B cells CD19 Antibody, clone FMC63 is validated for use in FC, IF for the detection of CD19.
This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry and immunofluorescence.
SUGGESTED USAGE DILUTION
Flow cytometry and Indirect Immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Biochem/physiol Actions
This specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma.
Cell reactivity:
Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes.
Clinical:
B cell chronic lymphocytic leukemia (B-CLL) +
Pro lymphocytic leukemia (PLL) +
Hairy cell leukemia (HCL) +
Common acute lymphoblastic leukemia (CALL) +
Pre-B acute lymphoblastic leukemia (pre-B-ALL) +
NULL-acute lymphoblastic leukemia (NULL-ALL) +
T common lymphocytic leukemia (T-CLL) -
Physical form
Format: Purified
Purified from mouse ascitic fluid. The antibody is supplied in 25 mM Tris-HCl buffer, 0.4 M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Preparation Note
Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL141
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
