Description
General description
116 kDa calculated
Integrin alpha-V (UniProt P06756; CD51, Integrin alpha V, Vitronectin receptor subunit alpha) and Integrin beta-3 (UniProt P05106; CD61, GPIIIa, Platelet membrane glycoprotein IIIa, Vitronectin receptor subunit beta) are encoded by the ITGAV (MSK8, VNRA, VTNR; Gene ID 3685) and the ITGB3 (GP3A; Gene ID 3690) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor alphaVbeta3 (also known as vitronectin receptor or VnR) appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue. Due to its involvement in angiogenesis, integrin alphaVbeta3 is among of the most studied integrin receptors.
Immunogen
Epitope: Extracellular domain
Application
Anti-Integrin αVβ3 Antibody, clone 27.1 (VNR-1) (Azide Free) is an antibody against Integrin αVβ3 for use in Immunohistochemistry (Paraffin), Flow Cytometry and Affects Function.
Flow Cytometry Analysis: A representative lot detected αvβ3 expression on SKOV3, OVCAR3, and IGROV1 human ovarian cancer cells. The surface expression was shown to be downregulated following treatment with SPARC (secreted protein acidic and rich in cysteine) from mouse parietal yolk sac (Said, N., et al. (2007). Am. J. Pathol. 170(3):1054–1063).
Flow Cytometry Analysis: A representative lot detected the surface expression of vitronection receptor (VnR) complex on CHO cells co-transfected with αv and either wild-type β3 or the Cam variant of Glanzmann′s thrombasthenia with the Asp119Tyr mutation (β3cam) (Loftus, J.C., et al. (1990). Science. 249(4971):915-918).
Affects Function: A representative lot blocked M21 human melanoma cells from adhering to fibrinogen (Fg), von Willebrand Factor (VWF), or fibronectin (FN) coated surface in a Integrin αvβ3-dependent manner (Pilch, J., et al. (2002). J. Biol. Chem. 277(24): 21930-21938).
Affects Function: A representative lot blocked metastatic human breast cancer cells from adhering to collagen I matrix (Felding-Habermann, B., et al. (2001). Proc. Natl. Acad. Sci. U. S. A. 98(4):1853-1858).
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
Biochem/physiol Actions
Reacts with the human integrin alphaVbeta3. Shown to be specific for alphaVbeta3 by Loftus JC et al (1990) Science 249:915-918.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgGκ antibody in buffer containing PBS, no azide
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Immunohistochemistry in rat cerebral cortex tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Integrin αVβ3 in rat cerebral cortex tissue.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
