Merck, MAB1998Z, Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free

Application
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free is validated for use in FC, IH, IP, FUNC for the detection of Integrin α2β1.
flow cytometry: 10 μg/mL

Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1

immunohistochemistry in frozen tissue sections: 5 μg/mL

Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.

Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.

Final working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the collagen receptor VLA-2 (alpha2beta1) integrin complex.
Physical form
Format: Purified
Protein A purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing no preservative.
Preparation Note
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Skin, CD4 & CD8 positive T cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.