Merck, MAB2056, Anti-Integrin α3 Antibody, clone ASC-1

Immunogen
Human squamous cell carcinoma cells (SCC9)
Application
Anti-Integrin α3 Antibody, clone ASC-1 detects level of Integrin α3 & has been published & validated for use in FC, IP, IH.
Inhibition of cell attachment: inhibits SCC9 cell adhesion to human and

EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV.

Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC).

Immunoprecipitation: precipitates PAGE bands of 130kDa (reduced) or 116kDa and 135kDa (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells.

Working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
Biochem/physiol Actions
Human alpha3 integrin (CD49c)
Physical form
Format: Purified
Liquid in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL462B
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.