Merck, MAB2115, Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9

Immunogen
Human fetal liver cells
Application
Immunoprecipitation: 5μg/500μL of cell lysate in 0.5% triton X-100 diluted in PBS with proteinase inhibitors

Flow cytometry: 10μg/mL concentration; (1:100); per 10(e6) cells, 100μL sample volume.

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9 is validated for use in FC, IP for the detection of Cell Surface Antigen of Human Nucleated Erythroid Cells.
Biochem/physiol Actions
MAB2115 recognizes a 70 kDa surface protein in about 100% nucleated erythroid cells (erythroblasts, normoblasts, proerythroblasts), 36% of late erythroid committed cells and 70-80% of fetal liver cells. Normal stem cells, early erythroid committed cells and hematopoetic non-erythroid cells do not react with MAB2115. The 70 kDa protein may be associated with an isoform of transferrin specific for red cells. Applicable for (1) immunostaining and enumeration of nucleated erythroid cells in bone marrow, fetal liver or cord blood and (2) immunophenotyping human leukemias. {Mechetner, EB et al (1987) Identification of a human erythroid cell surface antigen by monoclonal antibody HAE9. Exp Hematol. 15(4):355-9}.
Physical form
Format: Purified
Purified immunoglobulin via gel filtration from tissue culture supernatants in 20mM PB pH 7.6, 250mM NaCL, with 0.1% sodium azide as a preservative.
Preparation Note
Maintain at 2-8°C for up to 6 months after date of receipt. The product may contain precipitation and microcentrifugation is recommended before use.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.