Merck, MAB4065, Anti-H-CAM Antibody, Pan, clone SFF-2

Immunogen
Epitope: Pan
Application
Immunohistochemistry: 1 µg/mL; acetone fixed cryostat sections; 2 µg/mL following heat-induced epitope retrieval in 0.01 M citrate buffer, pH 6.0 (Banfalvi, 1999).
Immunofluorescence
Flow Cytometry: 1:20 as determined on U937 cells
Optimal working dilutions must be determined by end user.

Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-H-CAM Antibody, Pan, clone SFF-2 is validated for use in FC, IC, IH, IH(P) for the detection of H-CAM.
Biochem/physiol Actions
Specific for human standard CD44. Does not distinguish between splice variants (exons v3v10).
Physical form
Format: Purified
Liquid in PBS.
Preparation Note
Prior to opening maintain at -20°C until expiration date. After opening maintain frozen at -20°C in undiluted aliquots until expiration date. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
POSITIVE CONTROL: Lymph node (lymphocytes), skin (keratinocytes), or tonsil.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1123
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.