Merck, MAB4120, Anti-MDR1 Antibody, clone JSB-1

General description
170 kDa
Immunogen
Epitope: Cytoplasmic
Application
Anti-MDR1 Antibody, clone JSB-1 detects level of MDR1 & has been published & validated for use in FC, IC, IH(P) & WB.
Flow Cytometry

Immunohistochemistry on frozen and paraffin embedded tissue

sections: 1:20.

Immunocytochemistry: acetone or air-dried preparations react well.

Western blot

Note: For cellular detection, permeabilization is required.

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Toxicology & Drug Resistance
Biochem/physiol Actions
Reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170 kDa P-gp, member of the superfamily of transmembrane transporters. JSB-1 detects P-gp overexpression in human tumor cells of all different histogenetic derivations.

JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
Physical form
UnPurified mouse culture supernatant containing 0.7% BSA and 0.1% sodium azide as a preservative.
Unpurified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
MDR cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.