Description
General description
36 kDa
TS (EC:2.1.1.45), a cytosolic enzyme, is a dimmer of two identical monomers of about 36kDa. The enzyme provides the sole intracellular de novo source of thymidylate and plays a crucial role in DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dump) and its conversion to deoxythymidine monophosphate (dTMP). Therefore, TS is primarily active in proliferating and metabolic active cells. TS is a central target of the widely used antineoplastic agent 5-Fluorouracil (5-FU) and thus also of the Xeloda, which is enzymatically activated to 5-FU. TS is inactivated by a covalent complex formation with 5-FdUMP and methylenetetrahydrofolate. Literature indicates that expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas with low TS expression predicting better response to 5-FU and survival.
Immunogen
Recombinant Thymidylate Synthase.
Application
ELISA:
A previous lot of this antibody was used in ELISA.
Flow Cytometry:
A previous lot of this antibody was used in FC.
Immunofluorescence:
A previous lot of this antibody was used in IF.
Immunoprecipitation:
A 10 μL/mg concentration of a previous lot was used on protein lysate.
Immunohistochemistry (Frozen and Paraffin sections):
1:50 - 1:100 (with incubation for 30-60 minutes at room temperature). TS106 can benefit from antigen retrieval with either EDTA pH 8.0 or 10mM citrate buffer pH 6.0 .
Western Blotting:
1:200 - 1:400. Recognizes a 36KDa band.
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Thymidylate Synthase Antibody, clone TS106 (Mouse Monoclonal Antibody) validated in ELISA, FC, IF, IHC, IHC(P), IP, WB to detect Thymidylate Synthase also known as Thymidylate synthase.
Biochem/physiol Actions
Antibody MAB4130 recognizes a 36 kDa protein identified as Thymidylate Synthase [TS], which is a target for the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. Expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas.
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in 0.02M PBS with 0.25M NaCl, pH=7.6, 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10
Evaluated by Western Blot on MCF7 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected THYMIDYLATE SYNTHASE on 10 μg of MCF7 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
