Merck, MABC1598-100UL, Anti-BAK Antibody, clone 7D10

General description
GST-tagged recombinant fragment corresponding to the first 186 amino acids of human BAK.
~24 kDa observed; 23.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Application
Induces Function Analysis: A representative lot induced conformational change and oligomerization of BAK and caused cytochrome c release. (Iyer, S., et. al. (2016). Nat Commun. 7:11734).

Immunoprecipitation Analysis: A representative lot immunoprecipitated BAK in Immunoprecipitation applications (Iyer, S., et. al. (2016). Nat Commun. 7:11734).

Western Blotting Analysis: A representative lot detected BAK in Western Blotting applications (Dewson, G., et. al. (2009). Mol Cell. 36(4):696-703; Kotschy, A., et. al. (2016). Nature. 538(7626):477-482; Alsop, A.E., et. al. (2015). Nat Commun. 6:6841).

Biochem/physiol Actions
Clone 7D10 is a rat monoclonal antibody that detects BAK protein in human and murine species. It binds close to alpha1 region in non-activated BAK.

Physical form
Purified rat monoclonal antibody IgG2a in PBS without azide.

Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in wild type and Bax/Bak double knockout HeLa cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BAK in Wild type HeLa cell lysate, but not in Bax/Bak double knockout HeLa cell lysate.

Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.