Merck, MABC680, Anti-AXL, clone 7E10 Antibody

General description
Purified recombinant extracellular fragment of human AXL fused with hIgGFc tag expressed in HEK293 cell line.
~67 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Application
Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected AXL in methanol-fixed HEK293 cells transfected with AXL-hIgGFc.

Physical form
Mouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in extracellular domain of human AXL.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected AXL in the extracellular domain.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.