Merck, MABC697, Anti-ACLY, clone 5F8D11 Antibody

General description
ATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.
~125 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human ACLY expressed in E. Coli
Application
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ACLY in esophageal cancer tissue.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ACLY in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ACLY in HeLa cells.

ELISA: A 1:10,000 dilution from a representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ACLY in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.