Merck, MABC975, Anti-Arylsulfatase B Antibody, clone 10G6.1

General description
GST-tagged recombinant human Arylsulfatase B N-terminal fragment.
~46 kDa observed. Target bands appear larger than the calculated molecular weights of 56.0 kDa (isoform 1) and 42.3 kDa (isoform 2) due to glycosylation.
Application
Epitope: N-terminal region.
Biochem/physiol Actions
Expected to react with both spliced isoforms of human Arylsulfatase B reported by UniProt (P15848-1 and P15848-2).
Physical form
Mouse monoclonal IgG2aκ antibody in tissue culture supernatant without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Arylsulfatase B in 10 µg of HepG2 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.