Merck, MABD122, Anti-CD73 Antibody, clone AA60-E3-3

General description
63 kDa calculated
CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.
Immunogen
Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)
Application
Detect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
This antibody has been shown to work in immunocytochemical applications on human adipose mesenchymal stem cells. This antibody has been shown to work in western blot applications on HMEC-1 cells by an independant lab.This antibody has been shown to work in immunoprecipitation applications on HMEC-1 cells by an independant lab.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human Adipose Mesenchymal Stem Cells
Evaluated by Flow Cytometry on human adipose mesenchymal stem cells.
Flow Cytometry Analysis: 1 µg/mL of this antibody detected CD73 on 1X10^6 human adipose mesenchymal stem cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABC668
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.