Merck, MABE1011, Anti-DNA Ligase 3 Antibody, clone 7H6.1

General description
GST-tagged recombinant protein corresponding to the C-terminal of human DNA Ligase 3.
~113/106 kDa observed. 112.9 kDa (isoform 1), 106.0 kDa (isoform 2), 102.7 kDa (isoform 3), 95.8 kDa (isoform 4) calculated.

Application
Epitope: C-terminal
Biochem/physiol Actions
Expected to react with all four spliced isoforms.
Physical form
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA Ligase 3 in 10 µg of HeLa nuclear extract.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.