Description
General description
5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.
Detects methylated cytosine
Immunogen
Ovalbumin-conjugated 5-methylcytosine
Application
IF
Habib, M., et al. (1999). Exp Cell Res. 249:46-53
IHC-P
Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693
Flow Cytometry
Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262
MeDIP
Keshet, I., et al. (2006). Nat Genet. 38(2):149-153
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Use Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine.
Biochem/physiol Actions
Predicted to react with multiple species.
This antibody detects 5-Methylcytosine, but not unmethylated cytosine.
Physical form
Format: Purified
Protein A
Purified mouse monoclonal IgG1λ in buffer containing PBS and 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Methylated and unmethylated BRCA1
Evaluated by Dot Blot in methylated and unmethylated BRCA1.
Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.
Other Notes
Replaces: MABE527
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
