Merck, MABF1521, Anti-mouse IFN gamma, violetFluor® 450, clone XMG1.2 Antibody

General description
The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. Other formats can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of IFN-g in a variety of assays (use format suitable for functional assays).
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Anti-mouse IFN gamma, violetFluor® 450, clone XMG1.2 Antibody is an antibody against mouse IFN gamma for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Physical form
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected IFN gamma, violetFluor® 450 in one million C57Bl/6 splenocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
violetFluor is a registered trademark of Tonbo Biotechnologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.