Merck, MABF714, Anti-CD54 (ICAM-1) Antibody (human), PE, clone 15.2

General description
The 15.2 antibody reacts with human CD54, also known as ICAM-1 (Intercellular Adhesion Molecule 1), a 90-110 kDa cell surface glycoprotein that is inducibly expressed on both immune and endothelial cells. As its name implies, ICAM-1 participates in cell-cell adhesion between leukocytes and endothelial cells, facilitating leukocyte recruitment and transmigration at sites of inflammation. The ligands for ICAM-1 are also expressed on leukocyte and endothelial cells, and include Mac-1, fibrinogen, and a member of the integrin protein family, LFA-1 (CD11a).The 15.2 antibody may be used for analysis of ICAM-1 expression in human cells and tissues, and is reported to be cross-reactive with porcine ICAM-1.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD54 (ICAM-1) (human), PE, clone 15.2 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD54 (ICAM-1).
Physical form
Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, <0.2% BSA, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Preparation Note
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD54 (ICAM-1) in one million human peripheral blood monocytes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.