Merck, MABF927, Anti-CD99 Antibody, clone 0662

General description
Human thymocytes.
~31 kDa observed.
Application
Epitope: Extracellular.
Biochem/physiol Actions
Clone 0662 detects both the 32 kDa glycosylated (plasma membrane) and the 28 kDa non-glycosylated (intracellular) forms of human CD99/E2 (Gelin, C., et al. (1989). EMBO J. 8(11):3253-3259). Note that the clone was originally designated as 0662 (zero six six two) by Bernard, A., et al. (1988), but subsequently cited in some publications as O662 with a captial letter O.
Physical form
Purified mouse monoclonal IgG3κ antibody in PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD99 in 10 µg of THP-1 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.