Merck, MABS1927, Anti-CD173 and CD174 Antibody, clone BRIC 231

General description
59.08 kDa calculated.
CD173 (also known as fucosylated histo-blood group antigens H type 2; Blood group O) and CD174 (also known as Lewis Y or LeY) are developmentally regulated cell surface carbohydrate antigens that are expressed to varying degrees on many human carcinomas. They are also expressed on CD34+ hematopoietic progenitor cells, but are absent on mature lymphocytes. Hence, it is suggested that CDS173 and CD174 play a role in homing process of hematopoietic stem cells. Fucosylated glycans are known to be involved in cell-cell and cell-matrix interactions, differentiation, cell proliferation, and apoptosis. CD173 is a monofucosylated H2 where as CD174 is a difucosylated LeY. They are structurally related and have 2 precursor chains (Galbeta1-4GlcNAc-) in common. CD173 is synthesized through the addition of a fucose on type 2 precursor chains by the alpha 1-2 fucosyltransferases. CD174 results from the epistatic interaction on type 2 precursor chains, of the products of the alpha 1-2 fucosyltransferases and alpha 1-3 fucosyltransferases.

Immunogen
Whole HEL cells

Application
Anti-CD173 CD174, clone BRIC 231, Cat. No. MABS1927, is a mouse monoclonal antibody that detects CD173/CD174 in human and is tested for use in Flow cytometry and Immunocytochemistry.
Immunocytochemistry Analysis: A representative lot detected CD173 CD174 in Immunocytochemistry applications (Cao, Y., et. al. (2001). Glycobiology. 11(8):677-83).

Flow Cytometry Analysis: A representative lot detected CD173 CD174 in Flow Cytometry applications (Cao, Y., et. al. (2001). Glycobiology. 11(8):677-83).

Research Category
Signaling
Biochem/physiol Actions
Clone BRIC231 detects CD173/CD174 in human cells.

Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note
Stable for 1 year at 2-8°C from date of receipt.

Analysis Note
Evaluated by Immunocytochemistry in KG1 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected CD173 CD174 in KG1 cells.

Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.