Merck, MABS815, Anti-ERK3, clone 4E8 Antibody

General description
Purified recombinant fragment of human ERK3 expressed in E. Coli.
~54 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Application
Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected ERK3 in HeLa cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Physical form
Mouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in HEK293 and ERK3-hIgGFc transfected HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected ERK3 in transfected HEK293 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.