Description
General description
The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue.
Clone Ab62 is a monoclonal antibody that specifically recognizes the LIBS2 epitope in the C-terminal region of the extracellular domain of the beta3 subunit (Wiberzbicka-Patynowski, I., et al. (1999). JBC. 274(53):37809-37814).
~110 kDa observed in human platelets.
Other MW bands may vary or may be observed depending on lysate used and if the LIBS2 epitope is present in other proteins.
Immunogen
Epitope: LIBS2 epitope in the C-terminal region of the extracellular domain of the beta3 subunit of integrins.
Human GPIIa-IIIb purified from platelets
Application
Anti-LIBS2 epitope (Integrin beta3 subunit) Antibody, clone ab62 is an antibody against LIBS2 epitope (Integrin beta3 subunit) for use in WB, FC.
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Ginsberg, M., et al. (1990). Blood. 76:2017-2023.)
Research Category
Cell Structure
Research Sub Category
Integrins
Biochem/physiol Actions
This antibody recognizes the LIBS2 epitope in the C-terminal region of the extracellular domain of the beta3 subunit of integrins.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human platelets cell lysate
Evaluated by Western Blot in human platelets cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected LIBS2 domain of integrin beta3 subunit in 10 µg of human platelets cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
