Description
General description
The carcinoembryonic antigen- (CEA-) related cell adhesion molecules (CEACAMs) constitute a 12-member subgroup of the CEA family of immunoglobulin-related proteins first described in 1965 (PMID 4953873). CEACAMs are reported to participate in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEACAM molecules from neighbouring cells can interact via their respective extracellular N-terminal IgV-like domain and mediate cell-cell adhesion through trans-oligomerization. CEACAM molecules within the same cell can also undergo transmembrane domain-mediated cis-oligomerization, an event important for sustaining downstream cellular signaling. Athough CEACAM1 is shown to utilize its cytoplasmic domain for transducing cellular signaling, not all CEACAM members are expressed with a significant cytoplasmic domain, and others (CEACAM5/6/7/8) are GPI-anchored without even a transmembrane domain. (PMID 4735478). Rodent Ceacam1 (also known as C-CAM 105, Cell-CAM 105, Ecto-ATPase, GP110, pp120; UniProt P16573) is encoded by the Ceacam1 gene (NCBI Gene ID 81613). As is the case with its human counterpart (P13688), there exisit a long (canonical; 519aa) and a short (458aa) form with a reduced cytoplamic domain as a result of alternate splicing. An additional 10 splice variants are also reported for human CEACAM1. Clone 11-1H does not cross-react with human CEACAM1/3/4/5/6/7/8 or mouse CEACAM1/2.
Immunogen
Recombinant protein corresponding to the extracellular, excluding N domain of rat CEACAM1.
Application
Anti-CEACAM1 Antibody, clone 11-1H is an antibody against CEACAM1 for use in Western Blotting, Immunohistochemistry, ELISA, Flow Cytometry and Immunoprecipitation.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected CEACAM1 in rat liver, lung and kidney tissue.
ELISA Analysis: A representative lot detected CEACAM1 in CHO-cells expressing CEACAM1s (Dr. B. Singer, University Duisb
Analysis Note
Evaluated by Western Blotting in rat liver tissue lysate.
Western Blotting Analysis: 0.5 ug/mL of this antibody detected CEACAM1 in 10 ug of rat liver tissue lysate.
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
