Merck, MABT520, Anti-Cytokeratin 18, clone 4D11E4 Antibody

General description
Purified recombinant fragment of human Cytokeratin 18 expressed in E. Coli.
~62 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Application
Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.

Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected Cytokeratin 18 in human breast carcinoma, hepatocarcinoma, stomach cancer, and colon cancer tissues.
Physical form
Mouse monoclonal IgG2bascitic fluid containing 0.03% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting using cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.