Description
General description
GST (glutathione-S-transferase) represents a major group of detoxification enzymes, which are known to exist as multiple cytosolic and membrane-bound isoenzymes, with distinct catalytic and noncatalytic binding properties. Whereas the cytosolic enzymes are encoded by at least five distantly related gene families, the membrane-bound enzymes are encoded by single genes. GST is regulated and induced by a diverse range of xenobiotics. Many, but not all, inducers of GST, act through either the antioxidant-responsive element (ARE), the GST P enhancer 1(GPE), the xenobiotic-responsive element (XRE), or the glucocorticoid-responsive element (GRE). Barbiturates may transcriptionally activate GST through a Barbie box element. Many of the compounds that induce GST are also GST substrates, or are metabolized by cytochrome P-450 monooxygenases into compounds that can serve as GST substrates. Because the expression levels of GST play a critical role in determining the sensitivity of cells to a broad range of toxic chemicals, GST is suggested to contribute to resistance against carcinogens, antitumor drugs, environmental pollutants, and products of oxidative stress.
~45 kDa observed. The molecular weight of the GST•Tag fusion protein is not ~45 kDa The Posi-Tag Epitope Tag Control, which contains the fusion protein, is observed at ~45 kDa in Western Blot.
Immunogen
Gluththione-S-transferase (GST) domain from Schistosoma Japonicum
Application
Immunocytochemistry Analysis: A representative lot detected GST•Tag fusion protein in transfected COS-1 cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated GST•Tag fusion protein sequence in IP.
ELISA Analysis: A representative lot detected GST•Tag protein sequence in ELISA.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
This Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Posi-Tag Epitope Tag Control
Evaluated by Western Blot in Posi-Tag Epitope Tag Control.
Western Blot Analysis: This antibody detected GST•Tag fusion protein in 5 µL of Posi-Tag Epitope Tag Control.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
