Merck, MAK110-1KT, MTP Activity Assay Kit

General description
Microsomal triglyceride transfer protein (MTP), a membrane-bound protein present in the liver, plays an important role in the assembly and secretion of very low density lipoprotein (VLDL). MTP is also necessary for assembly and secretion of chylomicrons by the intestine. This protein facilitates the transfer of triglyceride (TG), cholesteryl ester (CE) and phosphatidylcholine (PC) between phospholipid surfaces. Mutation in the MTP gene leads to abetalipoproteinemia.
Application
Suitable for measuring microsomal [triglyceride] transfer protein (MTP) activity in cell lysates or tissue homogenates, or for screening MTP activity inhibitors.
MTP Activity Assay Kit has been used to measure the activity of microsomal triglyceride transfer protein (MTP).
Biochem/physiol Actions
The MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate. This kit includes proprietary substrates that enable the detection of MTP-mediated transfer of neutral lipid. The transfer activity results in an increase in fluorescence intensity (λEx=465 nm/λEm=535 nm).
Features and Benefits
Compatible with high-throughput handling systems.
Analysis Note
Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.