Merck, MAK113-1KT, ATPase/GTPase Activity Assay Kit

General description
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.
Application
ATPase/GTPase Activity Assay Kit has been used to measure the ATPase enzyme activity in:

• Dicer-like 1 (DCL1) and its mutants, to terminate the reaction and generate a colorimetric product
• various brain regions of mice, facilitating the quantification of inorganic phosphate released from ATP
• cell lysates, for the development and encapsulation of the reconstituted cell-free enzyme system

Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.
Biochem/physiol Actions
The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.
Features and Benefits
Compatible with high-throughput handling systems.