Merck, MAK127-1KT, Copper Assay Kit

General description
Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin, and collagen. Copper is mainly present in ceruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anemia, hypotonia, and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson′s disease, microcytic hypochromic anemia, and bone disease due to reduced collagen synthesis.
Application
Copper Assay Kit has been used to determine the copper content colorimetrically in samples.
Suitable for the detection of of copper in biological, environmental, food, and beverage samples. Suitable for studying the effects of compounds on copper metabolism.
Biochem/physiol Actions
The method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured colorimetrically (359 nm), is directly proportional to copper concentration in the sample. The range of linear detection is 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM).
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.